Extracellular idiotypic immunoglobulin arising from human leukemic B lymphocytes.

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Extracellular idiotypic immunoglobulin arising from human leukemic B lymphocytes

The peripheral blood lymphocytes of nine out of nine patients with typical surface Ig-positive chronic lymphocytic leukemia but no paraprotein visible on serum electrophoresis have been shown by radioimmunoassay to export small amounts of pentameric IgM during culture (in the range of 2.4-7.2 ng/10(7) cells per h); three out of nine also exported monomeric IgD (0.7-1.4 ng/10(7) cells per h). Im...

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Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes

Successful induction of in vitro differentiation and immunoglobulin synthesis of the leukemic lymphocytes was carried out in two cases of chronic lymphocytic leukemia. Few plasma cells and little specific Ig secretion were detected in the cultures of isolated leukemic B cells in either the presence or the absence of autologous T cells. Up to 30% of the leukemic B cells matured to plasma cells, ...

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Cytogenetic and immunoglobulin markers of human leukemic B-cell lines.

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Idiotypic Determinants of Immunoglobulin M Detected on the Surface of Human Lymphocytes by Cytotoxicity Assays

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The export of immunoglobulin D by human neoplastic B lymphocytes

An investigation has been made into the ability of human neoplastic B lymphocytes expressing surface IgM and IgD to export IgD in culture. Cells that expressed surface Ig of the lambda light chain type frequently exported IgD (10/12 patients), whereas cells expressing surface Ig of the kappa light chain type exported no IgD, although most (8/11 patients) were able to export IgM. It appears, the...

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1980

ISSN: 0022-1007,1540-9538

DOI: 10.1084/jem.152.6.1484